LOCUS       sequence-53241-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FDX1
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             411..431
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             432..449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             450..473
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(593..611)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      607..654
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(778..797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atattttgtt taccttaaga aggagatata ctatgagcag ctcagaagat aaaataacag
       61 tccactttat aaaccgtgat ggtgaaacat taacaaccaa aggaaaagtt ggtgattctc
      121 tgctagatgt tgtggttgaa aataatctag atattgatgg ctttggtgca tgtgagggaa
      181 ccctggcttg ttcaacctgt cacctcatct ttgaagatca catatatgag aagttagatg
      241 caatcactga tgaggagaat gacatgctcg atctggcata tggactaaca gacagatcac
      301 ggttgggctg ccaaatctgt ttgacaaaat ctatggacaa tatgactgtt cgagtgcctg
      361 aaacagtggc tgatgccaga caatccattg atgtgggcaa gacctccgca gagaacctct
      421 acttccaatc gcaccatcat caccaccatg attacaagga tgacgacgat aagtgaggat
      481 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      541 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      601 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      661 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      841 atcggggggc tccctttagg gttccgattt agtgctttac ggcacctcga cccccaaaaa
      901 aacttgatta gggtg
//
