LOCUS       sequence-53244-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PMP2
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             546..563
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(616..634)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      630..677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tactttagag gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg
       61 ggtaccgaga acctgtactt ccaatccatg aacaaattcc tgggcacctg gaaacttgtc
      121 tctagtgaga actttgacga ttacatgaaa gctctgggtg tggggttagc caccagaaaa
      181 ctgggaaatt tggccaaacc cactgtgatc atcagcaaga aaggagatat tataactata
      241 cgaactgaaa gtacctttaa aaatacagaa atctccttca agctaggcca ggaatttgaa
      301 gaaaccacag ctgacaatag aaagaccaag agcatcgtaa ccctgcagag aggatcactg
      361 aatcaagtgc agagatggga tggcaaagag acaaccataa agagaaagct agtgaatggg
      421 aaaatggtag cggaatgtaa aatgaagggc gtggtgtgca ccagaatcta tgagaaggtc
      481 taacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac
      541 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      601 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      661 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      721 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      781 tgccagcgcc ctagcgcccg ctccctttcg ctttcttccc ttcctttctc gccacgttcg
      841 ccggctttcc ccgtcaagct ctaaatccgg ggggctccct ttagggttcc cgattttagt
      901 gctttacggc acctcgaccc caaaaaactt gattaggggt gatggt
//
