LOCUS       sequence-53245-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RAMP2
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             445..462
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(515..533)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      529..576
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      613..971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(700..719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     910..931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgacaggca caccagggtc
      121 agaagggggg acggtgaaga actatgagac agctgtccaa ttttgctgga atcattataa
      181 ggatcaaatg gatcctatcg aaaaggattg gtgcgactgg gccatgatta gcaggcctta
      241 tagcaccctg cgagattgcc tggagcactt tgcagagttg tttgacctgg gcttccccaa
      301 tcccttggca gagaggatca tctttgagac tcaccagatc cactttgcca actgctccct
      361 ggtgcagccc accttctctt gacagtaaag gtggatacgg atccgaattc gagctccgtc
      421 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      481 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      541 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      601 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      721 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      781 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      841 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      901 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      961 cttttgattt ataagggat
//
