LOCUS       sequence-53246-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CDC25C
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             751..768
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(822..840)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgactcag atgctggagg
      121 aagattctaa ccaggggcac ctgattggtg atttttccaa ggtatgtgcg ctgccaaccg
      181 tgtcagggaa acaccaagat ctgaagtatg tcaacccaga aacagtggct gccttactgt
      241 cggggaagtt ccagggtctg attgagaagt tttatgtcat tgattgtcgc tatccatatg
      301 agtatctggg aggacacatc cagggagcct taaacttata tagtcaggaa gaactgttta
      361 acttctttct gaagaagccc atcgtccctt tggacaccca gaagagaata atcatcgtgt
      421 tccactgtga attctcctca gagaggggcc cccgaatgtg ccgctgtctg cgtgaagagg
      481 acaggtctct gaaccagtat cctgcattgt actacccaga gctatatatc cttaaaggcg
      541 gctacagaga cttctttcca gaatatatgg aactgtgtga accacagagc tactgcccta
      601 tgcatcatca ggaccacaag actgagttgc tgaggtgtcg aagccagagc aaagtgcagg
      661 aaggggagcg gcagctgcgg gagtaagaca gtaaaggtgg atacggatcc gaattcgagc
      721 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      781 gctaacaaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      841 ataacccctt ggggcctcta aacggg
//
