LOCUS       sequence-53249-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PKMYT1
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg ggtaccgaga
       61 acctgtactt ccaatccatg caccagttgc aacctcgtcg tgtttctttt cgcggcgagg
      121 cttcagaaac cttgcagagc ccaggttatg atccgtcccg cccagaaagt ttctttcaac
      181 agtcgttcca acgcctcagc cgtctgggac atggttctta cggcgaagta ttcaaagtac
      241 gctccaaaga ggacggccgt ttgtatgcgg tgaaacgctc aatgagtccg tttcgtggcc
      301 ccaaagatcg cgcccgtaag ctggcggaag ttggctctca cgagaaagtc ggccagcatc
      361 cgtgttgcgt acgcctggag caagcctggg aagaaggcgg tattctgtac ctgcagacag
      421 agctgtgcgg cccttcactg caacagcact gtgaggcgtg gggtgcaagt ttgccagaag
      481 ctcaagtgtg gggttatctg cgtgacacct tgctggcctt ggcacatctg cattcgcagg
      541 gtttggttca cctggatgtc aagcccgcca acatcttcct gggccctcgt ggtcgttgta
      601 aactgggcga ttttggcctg ttggtggagt tgggcactgc gggtgctggt gaagtacaag
      661 aaggcgatcc gcgttacatg gcaccagaat tgctgcaggg ctcttatggt acagccgcgg
      721 atgttttcag cctgggcttg actattctgg aggtagcttg caatatggag ctgccccacg
      781 gcggcgaagg ttggcaacag ttgcgccaag gttacctgcc ccctgagttt accgccggtt
      841 tgtcatccga gttgcgcagt gtactggtca tgatgctgga gcccgatccg aaactgcgcg
      901 caacagctga agcattgctg gctttgcccg ttttgcgcca acc
//
