LOCUS       sequence-53250-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LCN15
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             656..673
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(726..744)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      740..787
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccctctaga atattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgcaggctg
      121 aggttctgct gcagcctgac ttcaatgctg aaaagttctc aggcctctgg tacgtggtct
      181 ccatggcatc tgactgcagg gtcttcctgg gcaagaagga ccacctgtcc atgtccacca
      241 gggccatcag gcccacagag gagggcggcc tccacgtcca catggagttc ccgggggcgg
      301 acggctgtaa ccaggtggat gccgagtacc tgaaggtggg ctccgaggga cacttcagag
      361 tcccggcctt gggctacctg gacgtgcgca tcgtggacac agactacagc tccttcgccg
      421 tcctttacat ctacaaggag ctggaggggg ccctcagcac catggtgcag ctctacagcc
      481 ggacccagga tgtgagtccc caggctctga agtccttcca ggacttctac ccgaccctgg
      541 ggctccccaa ggacatgatg gtcatgctgc cccagtcaga tgcatgcaac taacagtaaa
      601 ggtggatacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca
      661 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      721 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      781 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      901 ctagcgcccg ctccttttcg ctttcttccc ttccttttct cgccacgttt cgccggcttt
      961 tccccgtcaa gctctaaatc ggggggcttc ccttta
//
