LOCUS       sequence-53252-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CLK2
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcctctag aatattttgt ttactttaga aggagatata catatgcacc atcatcatca
       61 tcattcttct ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgcgctcatc
      121 ttcgcacagc agccggagag ccaagagtgt agaggacgac gctgagggcc acctcatcta
      181 ccacgtcggg gactggctac aagagcgata tgaaatcgtt agcaccttag gagaggggac
      241 cttcggccga gttgtacaat gtgttgacca tcgcaggggt ggggctcgag ttgccctgaa
      301 gatcattaag aatgtggaga agtacaagga agcagctcga cttgagatca acgtgctaga
      361 gaaaatcaat gagaaagacc ctgacaacaa gaacctctgt gtccagatgt ttgactggtt
      421 tgactaccat ggccacatgt gtatctcctt tgagcttctg ggccttagca ccttcgattt
      481 cctcaaagac aacaactacc tgccctaccc catccaccaa gtgcgccaca tggccttcca
      541 gctgtgccag gctgtcaagt tcctccatga taacaagctg acacatacag acctcaagcc
      601 tgaaaatatt ctgtttgtga attcagacta tgagctcacc tacaacctag agaagaagcg
      661 agatgagcgc agtgtgaaga gcacagctgt gcgggtggta gactttggca gtgccacctt
      721 tgaccatgag caccatagca ccattgtctc cactcgccat taccgagcac cagaagtcat
      781 ccttgagttg ggctggtcac agccttgtga tgtgtggagt ataggctgca tcatctttga
      841 atactatgtg ggattcaccc tcttccagac ccatgacaac agagagcatc tagccatgat
      901 ggaaaggatc ttgggtccta tcccttcccg gatgatccga aagaca
//
