LOCUS       sequence-53255-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAST1
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             470..487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(540..558)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      554..601
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(725..744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaatattttg tttactttag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgcgtagcc
      121 cgattaccat tcagcgtagc ggcaaaaaat atggttttac cctgcgtgcg attcgcgtgt
      181 atatgggcga taccgatgtt tatagcgttc atcatattgt ttggcatgtg gaagaaggtg
      241 gtccggcgca ggaagcgggc ctgtgcgcgg gcgatctgat tacccatgtg aatggtgaac
      301 cggtgcatgg catggttcat ccggaagtgg ttgaactgat tctgaaaagc ggtaataaag
      361 tggcggtgac caccaccccg tttgaaaaca cccagagcct ggtttgacag taaaggtgga
      421 tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
      481 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      541 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      601 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      901 tcgccctttt gacgttggga gtccacgttc tttaatagtg gaactcttgt tccaaactgg
      961 aacaacactc aacccctatc tcgggtctat ttc
//
