LOCUS       sequence-53258-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_V1922D_V2074A_L2115W
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..93
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             118..771
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
ORIGIN
        1 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatata
       61 ggatctatgc accaccatca ccaccatcac cacgagaacc tttatttcca aggatcaatg
      121 gcaccgatcc tggggtattg gaagatcaag ggcctagtgc agcccactcg tctgctcctg
      181 gagtacctag aagagaagta tgaggagcac ctttatgagc gggacgaggg tgacaagtgg
      241 cgcaataaga aattcgagct aggcttggaa ttccccaatc tgccttatta catcgatggc
      301 gacgttaagt tgactcagtc catggccatc atccgctaca tcgccgacaa gcacaatatg
      361 ctaggcgggt gcccgaagga gcgagctgag atctctatgt tggaaggggc cgtgctggat
      421 atccgctacg gcgtgagccg cattgcatac tcaaaggatt tcgagaccct caaggttgac
      481 ttcctgtcaa agctgcctga aatgctaaag atgttcgaag accgtctctg tcataagacc
      541 tacctgaacg gggaccacgt gacacacccg gactttatgt tgtatgacgc tctggacgtc
      601 gtgctttata tggaccctat gtgcctggac gcttttccca aactagtgtg tttcaagaaa
      661 cgtatcgaag ctatccctca gattgataag tacctgaagt cctcaaagta catcgcctgg
      721 ccccttcaag gttggcaagc cacatttggc ggtggcgacc atcctcccaa gtcagaccta
      781 gtgcctaggc acaaccagac cagcctatat aagaaagccg ggtcagctgc cgctgtgctg
      841 gaggagaacc tgtatttcca g
//
