LOCUS       sequence-53262-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CECR2
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             509..526
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(579..597)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      593..640
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(764..783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gcgtgaagaa aaaaaaacca
      121 aagatctgtt tgaactggat gatgatttta ccgccatgta taaagtgctg gatgtggtga
      181 aagcccacaa agatagctgg ccgtttctgg aaccggtgga tgaaagctat gcgccgaatt
      241 attatcagat cattaaagcg ccgatggata ttagcagcat ggaaaaaaaa ctgaatggtg
      301 gcctgtattg caccaaagaa gaatttgtga acgatatgaa aaccatgttt cgtaactgcc
      361 gtaaatataa tggtgaaagc agcgaatata ccaaaatgag cgataacctg gaacgttgct
      421 ttcatcgtgc gatgatgaaa cattgacagt aaaggtggat acggatccga attcgagctc
      481 cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      541 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      601 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      661 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaact
//
