LOCUS       sequence-53265-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Kowalczyk SW, Kapinos L, Blosser TR, Magalhaes T, van Nies P, Lim 
            RY, Dekker C
  TITLE     Single-molecule transport across an individual biomimetic nuclear 
            pore complex.
  JOURNAL   Nat Nanotechnol. 2011 Jun 19;6(7):433-8. doi: 10.1038/nnano.2011.88.
  PUBMED    21685911
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nnano.2011"; journalName: "Nat Nanotechnol"; date: 
            "2011-06-19- 19"; volume: "6"; issue: "7"; pages: "433-8"
COMMENT     Sequence Label: pPEP-TEV cNup98 (1-498)
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             175..195
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggccacc atcaccatca tcacatgcaa
       61 aaatacttag aagataaagc tcaagaattg gtgcgactgg aaggagaggt tcgctccctc
      121 cttaaggaca taagtgagaa agttgcggtt tacagcaccg gcggtggtgg cggtgagaat
      181 ttatattttc aagggggatc ctgttgctgt tttaacaaat catttggaac accctttggg
      241 ggtggcacag gtggctttgg cacaacttca acatttggac agaatactgg ctttggcact
      301 actagtggag gggcatttgg aacatctgca tttggttcta gcaacaatac tggaggcctc
      361 tttggaaatt cacagactaa accaggagga ttgtttggaa ccagttcatt tagccagcca
      421 gctacctcca caagcactgg ctttgggttt ggtacgtcaa caggaacagc aaataccttg
      481 tttggaactg caagcacagg gaccagtctc ttctcatccc aaaacaatgc ctttgcacaa
      541 aataaaccaa ctggctttgg caattttgga accagtacta gcagtggagg actctttgga
      601 accacaaata ccacctctaa tccttttggc agcacatctg gctccctctt tgggccaagt
      661 agttttacag ctgctcctac tgggactact attaaattta accctccaac tggtacagat
      721 actatggtca aagctggagt tagcactaac ataagtacca agcaccagtg tattactgct
      781 atgaaagaat
//
