LOCUS       sequence-53276-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TGM1
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(323..343)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(368..385)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             396..418
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    433..457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(457..476)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(458..476)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(560..579)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctcgaattcg gatccgtatc cacctttact gttaggccac atccacctgg atgacaccgt
       61 gcacctggga gagctgtggg ctgtccaagc tggcaatgag ctggcggggg cctggtagca
      121 caggcacaaa cgactggcgc agtgtcactg tttcattgcc tccaatgtcc ccaacgttga
      181 ggatcttggg cctctgtaac ccagagcctt cgagccggaa gacgacattg gtgagggtga
      241 cgggaagggg gttcttgaag acaatctgta cttcacactc ctggccaacc actgctgctc
      301 ccagtaacgt gagggagagc atggattgga agtacaggtt ctcggtaccc agatctacac
      361 cagaagaatg atgatgatga tggtgcatat gtatatctcc ttcttaaagt taaacaaaat
      421 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      481 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      541 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atgggggaag atcgggctcg
      601 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      661 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg gtgctcaacg
      721 gcctcaaccc tactactgg
//
