LOCUS       sequence-53284-e         439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG268
FEATURES             Location/Qualifiers
     source          1..439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..95
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     87..104
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     88..104
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    155..179
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
       61 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcctgca
      121 ggtcgactct agaggatccg aattctgaaa tatcaccact ttgtacaaga aagctgggtc
      181 taatagtgtc ctcttagtcc gagattttcc tcagctgtcc acttcgtgct gagagatgtg
      241 cgacgcttgt tcatcggtgg attttctctg cgattccgtc tcaagtgtgg tggcaagtcg
      301 tcttcgttgg attccggttg attgtaatca cctccggctt gaagatcgcg accagcattg
      361 gagaggcaca tcattgagcc tgccacattt cgatgtttga gcatcacttt ggtcggcgtg
      421 gtagctcttc cacctgtac
//
