LOCUS       sequence-53293-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DHRS4
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt
       61 agatctgggt accgagaacc tgtacttcca atccatggcc agctccggga tgacccgccg
      121 ggacccgctc gcaaataagg tggccctggt aacggcctcc accgacggga tcggcttcgc
      181 catcgcccgg cgtttggccc aggacggggc ccatgtggtc gtcagcagcc ggaagcagca
      241 gaatgtggac caggcggtgg ccacgctgca gggggagggg ctgagcgtga cgggcaccgt
      301 gtgccatgtg gggaaggcgg aggaccggga gcggctggtg gccacggctg tgaagcttca
      361 tggaggtatc gatatcctag tctccaatgc tgctgtcaac cctttctttg gaagcataat
      421 ggatgtcact gaggaggtgt gggacaagac tctggacatt aatgtgaagg ccccagccct
      481 gatgacaaag gcagtggtgc cagaaatgga gaaacgagga ggcggctcag tggtgatcgt
      541 gtcttccata gcagccttca gtccatctcc tggcttcagt ccttacaatg tcagtaaaac
      601 agccttgctg ggcctgacca agaccctggc catagagctg gccccaagga acattagggt
      661 gaactgccta gcacctggac ttatcaagac tagcttcagc aggatgctct ggatggacaa
      721 ggaaaaagag gaaagcatga aagaaccctg cggataagaa ggttaggcga gccagaggat
      781 tgtgctggca tcgtgtcttt cctgtgctct gaagatgcca gctacatcac tggg
//
