LOCUS       sequence-53297-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ENO3
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             44..64
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcaccatcat catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca
       61 atccatggcc atgcagaaaa tctttgcccg ggaaatcttg gactccaggg gcaaccccac
      121 ggtggaggtg gacctgcaca cggccaaggg ccgattccga gcagctgtgc ccagtggggc
      181 ttccacgggt atctatgagg ctctggaact aagagacgga gacaaaggcc gctacctggg
      241 gaaaggagtc ctgaaggctg tggagaacat caacagtact ctgggccctg ctctgctgca
      301 aaagaaacta agcgttgcgg atcaagaaaa agttgacaaa tttatgattg agctagatgg
      361 gaccgagaat aagtccaagt ttggggccaa tgccatcctg ggcgtgtcct tggccgtgtg
      421 taaggcggga gcagctgaga agggggtccc cctgtaccgc cacatcgcag atctcgctgg
      481 gaaccctgac ctcatactcc cagtgccagc cttcaatgtg atcaacgggg gctcccatgc
      541 tggaaacaag ctggccatgc aggagttcat gattctgcct gtgggagcca gctccttcaa
      601 ggaagccatg cgcattggcg ccgaggtcta ccaccacctc aagggggtca tcaaggccaa
      661 gtatgggaag gatgccacca atgtgggtga tgaaggtggc ttcgcaccca acatcctgga
      721 gaacaatgag gccctggagc tgctgaagac ggccatccag gcggctggtt acccagacaa
      781 ggtggtgatc ggcatggatg tggcagcatc tgagttctat cgcaatggga agtacgatct
      841 tgacttcaag tcgcctgatg atcccgcacg gcacatcact g
//
