LOCUS       sequence-53299-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRWD1
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..48
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             525..542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(595..613)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      609..656
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtttacttt aagaaggaga tatacatatg caccatcatc atcatcattc ttctggtgta
       61 gatctgggta ccgagaacct gtacttccaa tccatggcga ccaactatgt ggaaagcaac
      121 tggaaaaaac agtgcaaaga actggtgaac ctgatttttc agtgcgaaga tagcgaaccg
      181 tttcgtcagc cggttgatct ggtggaatat ccggattatc gtgatatcat tgataccccg
      241 atggattttg gcaccgtgcg cgaaaccctg gatgccggca actatgatag cccgctggaa
      301 ttttgcaaag atattcgcct gatttttagc aacgcgaaag cctatacccc gaacaaacgc
      361 agcaaaatct atagcatgac cctgcgtctg agcgcgctgt ttgaagaaaa aatgaaaaaa
      421 attagcagcg attttaaaat tggtcagaaa tttaacgaat gacagtaaag gtggatacgg
      481 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      541 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      601 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      661 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      781 tcctttcgct ttcttcccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      841 taaatcgggg gcctcccctt tagggttccg a
//
