LOCUS       sequence-53302-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD2
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             444..461
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(514..532)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      528..575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(699..718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgggcaaact gagcgaacaa
       61 ctgaagcatt gcaatggcat tcttaaagag ctcctgagta aaaaacacgc cgcctatgcg
      121 tggccatttt ataaaccggt ggatgcctct gcgctgggtc tgcatgatta tcacgatatc
      181 attaaacatc cgatggatct ctcaaccgtt aaacgtaaaa tggaaaatcg cgattatcgt
      241 gatgcccagg aatttgcggc ggatgtacgc ctcatgtttt cgaactgcta caaatataac
      301 cctccagatc acgatgttgt ggcaatggca cgaaagctac aggatgtatt tgagttccgt
      361 tatgccaaga tgccagattg acagtaaagg tggatacgga tccgaattcg agctccgtcg
      421 acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      541 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      601 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      781 gtttccgaat ttagtggttt acggcacctt cgaccccaaa aaaacttgat tagggtgatg
      841 gttcacgtta atgggccaat cgccctggat agaacggttt tttcc
//
