LOCUS       sequence-53305-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: STK25
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..233
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             240..260
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atattttgtt tactttagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtggataa caagttcaac aaggagcgtc gaagagctcg ccgtgaaatt cgccatctgc
      121 cgaacctgaa ccgcgaacag cgtcgcgcat ttattcgcag cctgcgcgat gatccgagcc
      181 agagcgcgaa cctgctggcg gaagcgaaga agctgaacga tgcgcagccg aagggtaccg
      241 agaacctgta cttccaatcc atggctcacc tccggggatt tgccaaccag cactctcgag
      301 tggaccctga ggagctcttc accaagctcg accgcattgg caagggctcg tttggggagg
      361 tctacaaggg catcgataac cacacaaagg aggtggtggc catcaagatc atcgacctgg
      421 aggaggccga ggatgagatc gaggacatcc agcaggagat cactgtcctc agtcagtgcg
      481 acagccccta catcacccgc tactttggct cctacctaaa gagcaccaag ctatggatca
      541 tcatggagta cctgggcggc ggctcagcac tggacttgct taaaccaggt cccctggagg
      601 agacatacat tgccacgatc ctgcgggaga ttctgaaggg cctggattat ctgcactccg
      661 aacgcaagat ccaccgagac atcaaagctg ccaacgtgct actctcggag cagggtgacg
      721 tgaagctggc ggactttggg gtagcagggc agctcacaga cacgcagatt aagaggaaca
      781 cattcgtggg cacccccttc tggatggcac ctgaggtcat caagcagtcg gcctacgact
      841 tcaaggctga catctggtcc ctggggatca cagccatcga gctgg
//
