LOCUS       sequence-53306-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSP90B1
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             821..841
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             842..859
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             860..883
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             935..952
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaatattttg tttaccttaa gaaggagata tactatgaag accaagccta tttggaccag
       61 aaaccctgat gacatcaccc aagaggagta tggagaattc tacaagagcc tcactaatga
      121 ctgggaagac cacttggcag tcaagcactt ttctgtagaa ggtcagttgg aattcagggc
      181 attgctattt attcctcgtc gggctccctt tgaccttttt gagaacaaga agaaaaagaa
      241 caacatcaaa ctctatgtcc gccgtgtgtt catcatggac agctgtgatg agttgatacc
      301 agagtatctc aattttatcc gtggtgtggt tgactctgag gatctgcccc tgaacatctc
      361 ccgagaaatg ctccagcaga gcaaaatctt gaaagtcatt cgcaaaaaca ttgttaagaa
      421 gtgccttgag ctcttctctg agctggcaga agacaaggag aattacaaga aattctatga
      481 ggcattctct aaaaatctca agcttggaat ccacgaagac tccactaacc gccgccgcct
      541 gtctgagctg ctgcgctatc atacctccca gtctggagat gagatgacat ctctgtcaga
      601 gtatgtttct cgcatgaagg agacacagaa gtccatctat tacatcactg gtgagagcaa
      661 agagcaggtg gccaactcag cttttgtgga gcgagtgcgg aaacggggct tcgaggtggt
      721 atatatgacc gagcccattg acgagtactg tgtgcagcag ctcaaggaat ttgatgggaa
      781 gagcctggtc tcagttacca aggagggtct ggagctggca gagaacctct acttccaatc
      841 gcaccatcat caccaccatg attacaagga tgacgacgat aagtgaggat cccgaattcg
      901 agctccgtcg acaagcttgg cggccgcact cgagcaccac caccaccacc actgagatcc
      961 ggctggctaa caaagcccga aagggaagct ga
//
