LOCUS       sequence-53308-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSP90B1
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..90)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(91..108)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(109..129)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gtggtggtgg tggtgctcga gtgcggccgc atgaaggggt cgacggagct cgaattcgga
       61 tcctcactta tcgtcgtcat ccttgtaatc atggtggtga tgatggtgcg attggaagta
      121 gaggttctct gccagctcca gaccctcctt ggtaactgag accaggctct tcccatcaaa
      181 ttccttgagc tgctgcacac agtactcgtc aatgggctcg gtcatatata ccacctcgaa
      241 gccccgtttc cgcactcgct ccacaaaagc tgagttggcc acctgctctt tgctctcacc
      301 agtgatgtaa tagatggact tctgtgtctc cttcatgcga gaaacatact ctgacagaga
      361 tgtcatctca tctccagact gggaggtatg atagcgcagc agctcagaca ggcggcggcg
      421 gttagtggag tcttcgtgga ttccaagctt gagattttta gagaatgcct catagaattt
      481 cttgtaattc tccttgtctt ctgccagctc agagaagagc tcaaggcact tcttaacaat
      541 gtttttgcga atgactttca agattttgct ctgctggagc atttctcggg agatgttcag
      601 gggcagatcc tcagagtcaa ccacaccacg gataaaattg agatactctg gtatcaactc
      661 atcacagctg tccatgatga acacacggcg gacatagagt ttga
//
