LOCUS       sequence-53309-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG459
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    213..237
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
       61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcctgcagg
      181 tcgactctag aggatccgaa ttctgaaata tcaccacttt gtacaagaaa gctgggtccc
      241 taatagtgtc ctcttagtcc gagattttcc tcagatgtcc acttcgtgct gagagatgtg
      301 cgacgcttgt tcatcggtgg attttctctg cgattccgtc tcaagtgtgg tggcaagtcg
      361 tcttcgttgg attccggttg attgtaatca cctccggctt gaagatcgcg accagcattg
      421 gagaggcaca tcattgcgcc tgccacattt cgatgtttga gcatcacttt ggtcggcgtg
      481 gtagctcttc cacctgtacg catgaatcgt ggctgatgtc tagcgacaac gtagtcttct
      541 ggaatgacat cctggtcgta atcctggtca tcgtgagaca tgcgctgata tggaggaata
      601 ttctggaatt ccttgtccgt tggatgaaca aactcgcaac gaagtccata cgggcagaat
      661 ccagttcctc cacgcgcaaa gttcttgcac agctttgtct tgtacttgtt gttcggatag
      721 cgagccggag catcagtagc tctgagctct ttgagcccat gagcaaactt gcatcgagca
      781 cccatatcac atggtttgat tccagatgcg tgcatcatgc a
//
