LOCUS       sequence-53312-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACBD7
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(375..395)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(420..437)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             448..470
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    485..509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(509..528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(510..528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(612..631)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcgggctt gtagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc
       61 angcttgtcg acggagctcg aattcggatc cgtatccacc tttactgtca aattccgtat
      121 ttttctatca gctcctttgc tttagaaata taggcactcg tcgcatcttc cgtcgacaac
      181 ccttttttga ggttccatgc ttcccatttg gctttgcctt tcaaatctag cattcctgga
      241 cacgcaatat taatgtctcc aactattgct tgtttgtaaa gcccatagag ttctttcagt
      301 tctccatcat ctggtcttgc tttcagcttc ctcacatctt ctgcagccct gtcaaaatca
      361 gcctgcaggg ccatggattg gaagtacagg ttctcggtac ccagatctac accagaagaa
      421 tgatgatgat gatggtgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta
      481 gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat
      541 cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
      601 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gcccacttcg
      661 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg gcccc
//
