LOCUS       sequence-53315-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SCRIB
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(400..420)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(445..462)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             473..495
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    510..534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(534..553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(535..553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(637..656)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        862..941
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ctcttcggct tgtagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc aaacgctggt
      121 ttcacgttcg cgccagacac gcatttgcac ggcggtaccc gccccacgca gcgcttccac
      181 cgcctcatga tgctccgcgc cctgcagagc cacaccgtta acttccagca gtttatcccc
      241 aacgcggaca ccagcacgag cggccggacc ttcttcgcta acacgggaga taaaaatgcc
      301 ttcatcatcg cctttatacg gggtactacc tttgccgccg gcgatcgaaa tgcccaggcc
      361 gccggtctga cgcaggatgg tcagggtcag ttcttccatg gattggaagt acaggttctc
      421 ggtacccaga tctacaccag aagaatgatg atgatgatgg tgcatatgta tatctccttc
      481 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      541 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
      601 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
      661 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
      721 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
      781 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
      841 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca
      901 tggatagcgc ccgggaagag agtcaattca gggtggtgaa tgtgaaaacc agtaacgtta
      961 tacgat
//
