LOCUS       sequence-53316-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CA6
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actccttcgg cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgcctggaat tcagagccta
      181 gagtgtattc ctgattcggg aagttggatt ccaccactct gtggttcagg ggctgggtcc
      241 tgcggtaatc gttgtggatg gtcttgttgc ggtgatccag taaggaattc tccagcttcc
      301 aaacctgtgt cctggagagc ttgacaaaat ctgccagcac aaaccagtgg acgttctcag
      361 tgcagggagg cgtggtgagt gagccatggt aggtgtagta gtgctggagg ttcctgggca
      421 gcatgtcctg aacgtcaagg ccagtcaggg ttgttctttg tcctgggtac ttgatgttgg
      481 ccagatgaga aatgaagttg ctgtaataag tgttttcagg gtaattcttc acctcaacga
      541 aggctgccag tacagccaaa ccatccggcg catcttgggc tatatcatag ctcttgtatt
      601 tagaattgta gtgaacaatg tgaatctcga tcacatgtct gatcccgtcc acggtgtgct
      661 cagagccgct gatctccgag gacgcacctc cccagtgaaa gtgcatctgc tgggctatgt
      721 atacagtgcc gtcagccact gtcatgcgca tggtggaggg caggctgatc tgcactgtgt
      781 ggccattgtt gaccatgggg aactcccctg cctgggtctc atagcctgtc atattgagcc
      841 ccttcaagga ggggttgtac cgcaccttcg tcctctgta
//
