LOCUS       sequence-53319-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAGEA4
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(768..788)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(813..830)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             841..863
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    878..902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(902..921)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(903..921)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcagactccc
      121 tcttcctcct ctaacaaagc tgcttcacgc agggatgggt aggcaatgcg aactcttgca
      181 ttgaccctga ccacatgctc caggactttc acatagctgg tttcagccag agcccttgga
      241 ccccacagga actcatagcg cgcaggatta ctgccgggta cctgccggta ctccaggtag
      301 ttttcctgca cccaatcttg ggtgagcagt ttcctgggct ccccatagac agtgtgctcc
      361 ctcccatcat acacccccat cacacccagc tcctcccaga tttcctcctc agaggcgctg
      421 tcgccctcca ttgcaattgt gcccaggacg attatcagaa ggcctgtctt gggaaagatc
      481 tgattattac ccagcaggcc atcataggaa aggcccaggc aggtgacaag ggtgtaggtg
      541 ttgctggtgg ggtccacttc cttcacgtca atgccaaaga tcatcttcag ggactcggag
      601 gctttgccga agatcacagg aaagcagcgc ttgtaatttt tgatgactct ctccagcatt
      661 tctgcctttg tgaccagctc cttggctcga tacttgcgga gcagaaaatg agccaactca
      721 tccaccttgt tactgagtgc ttctcggaac aaggactctg cgtccatgga ttggaagtac
      781 aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg catatgtata
      841 tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt
      901 cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg
      961 catcgtggc
//
