LOCUS       sequence-53323-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX5
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(393..413)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(438..455)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             466..488
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    503..527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(527..546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(528..546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(630..649)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gacggagctc gaattcggat ccgtatccac ctttactgtc attcatctaa aagggcggag
       61 tggatcccca gctttttcag ttcttccacc aagtccccat tctggtgcat ctgcagaaga
      121 atgtcacagc cccctacaaa ctcgccattg aggtacactt gcgggatggt gggccagttg
      181 gaatagtctt taatgccttg tcggagctcc gggtcgtcca gcacgttgta ggccgcgtaa
      241 tcgcggacgc cgtgcagccg caggatctgc accacggcgt tgctgaagcc gcactggggc
      301 tgctccggcg tccccttgag gaagaccacc accttgtcct tcttcaccag cgcgtccaac
      361 tgctccgccg agccgccgcc gcccgcgccc atggattgga agtacaggtt ctcggtaccc
      421 agatctacac cagaagaatg atgatgatga tggtgcatat gtatatctcc ttcttaaagt
      481 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc
      541 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
      601 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atgggggaaa
      661 gatcgggctc gccactttc
//
