LOCUS       sequence-53324-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TXNRD3
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..28)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(47..67)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    435..459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(459..478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(460..478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(562..581)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctcacttatc gtcgtcatcc ttgtaatcat ggtggtgatg atggtgcgat tggaagtaga
       61 ggttctctgc atcataggcc aggtcttctt gcagcagttt ctgcagcagc ccactctgat
      121 atgcctgaaa agtctgatcg cagccaccga cgtgcacttt attcacgaaa atgttcggga
      181 ccgttttctg gttggtaatt tcagacagga cttcctgaac gcgggcacca tcatccacct
      241 ggtccagctc cagcacattg cattctacac ccaggctgga aaacagttct ttaacacgcg
      301 tactatgcgg acaataagac tttgaaaaga ttaccacacg ggagcgctca atcagtccaa
      361 ccagatgacg acgcagttct tcgcgcgcca tagtatatct ccttcttaag gttaaacaaa
      421 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      481 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      541 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      601 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      661 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      721 gcctcaacct actactgggg ctgcttccta atgcaggagt cgcataaggg agagcgtcgg
      781 agatcc
//
