LOCUS       sequence-53326-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACBD4
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(429..449)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(474..491)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             502..524
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    539..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(563..582)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(564..582)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(666..685)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcactctgcc
      121 acctcaccca ggggcactgt gtcgatcacc ttctgtgcca ccagtttcat ttcagtgatg
      181 taggcagaca tggcctcctc cctgctcatc ttgcccagac tgttccaggc gtcccactta
      241 tatcgtccaa tggggtccca gaacccgggc cgggggacca ggcagggccc catggtggcc
      301 tgcttgtagt aactgtagaa tcgcagcatc tcttcatagg aggggcggta agaaccgttc
      361 ttgggcaggt tctggatgac gctcactgca gcctggaact gtttctggca gtcgggctct
      421 gggctcatgg attggaagta caggttctcg gtacccagat ctacaccaga agaatgatga
      481 tgatgatggt gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
      541 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      601 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      661 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      721 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      781 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      841 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcc
//
