LOCUS       sequence-53327-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GAPDHS
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(90..113)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(114..131)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(132..152)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatcctcac ttatcgtcgt catccttgta atcatggtgg
      121 tgatgatggt gcgattggaa gtagaggttc tctgcgtctc ggctgaacat gtagcggagg
      181 aggtcgacca cccggtgact gtagccatat tcgttgtcgt accatgaaat gagcttcacg
      241 aaattgtcat tgagcgcaat gccggcctta gcatcgaaga tggacgagtg ggtatcaccg
      301 aggaagtccg tagagacgac ctcatcctcg gtgtaggcaa ggatgccagc catgggcccc
      361 ttggctgctg cttttacagc ctccttgatg gctgagtagg gggcaggctg ggcgaggcgg
      421 caggtcaggt ccacgacaga cacatccggg gttggtaccc ggaacgccat ccctgtcagc
      481 ttccctttga gctctgggat gactttggtc acagctttcg cagccccagt ggaggctggg
      541 atgatgttct ggtgggcacc ccgcccatct cgccaggcct tccttgatgg cccgtccact
      601 gtcttctggg tggccgtgta ggaatggact gtggtcatca acccttccac gatcccaaat
      661 cgctcgtgga tgactttggc gaggggagcc aaacagttgg tggtgcagga cgcgttgctc
      721 acaatgttca tggagccagg gttatagtca ttttcattga cacccatgac gaacattggt
      781 gcatccggtg agggcgcgga gatgaccaca cgttgagcac ctgcagagat gtggtccgaa
      841 gctgcctgta tggagaggta cacgcctgtg gactccacca cgta
//
