LOCUS       sequence-53328-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD2
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(86..109)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(110..127)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(128..148)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     RBS             207..215
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacggagctc gaattcggat cctcacttat cgtcgtcatc cttgtaatca tggtggtgat
      121 gatggtgcga ttggaagtag aggttctctg ccaataggtg ctgcagaatg tggtccacag
      181 catgagggaa actctcacaa gttaagtaag gaggtggatt aattttttct tcatctgatg
      241 ctcgatattt cccagtcttt actaagatgc ccagcatgcc gacatcttga gccccaccaa
      301 catcatccct gcaatcatct cctatcatga cagcctcctc aggttcacag ccagtgcccc
      361 gcaatgcttc caaaaagaac gtcttctctg gtttccccac gactgtggct ttggtatctg
      421 tggcatactc taaagcagtc acaaatggtc caggccccag ggctaagcca tctttcctct
      481 tgtaatacct ggctttgtgg attgctatca gaggtgctcc atccaggagt aaccggaatg
      541 cttgattcag aatttgataa tgaaaatgtt ctggtgccaa tcccatgacc acagcattag
      601 gatcacttgt ttgtattcct ttgaaatcag gtagtgcccg atcatcaact agcagcatgg
      661 gtctgacttg tttccgctct agtaaacttc tggctgcagt cagagatgtg aatatttcat
      721 cttcagagat atcaaattcc aattttctca acctttctaa caggtcttgc ttgctctctt
      781 tggttgtatt ggtcaca
//
