LOCUS       sequence-53329-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD9
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(414..434)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(459..476)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             487..509
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    524..548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(548..567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(549..567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(651..670)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttgtcgacg gagctcgaat tcggatccgt atccaccttt actgtcagct catgctgcgt
       61 ttcagggcca gcagacgttc tttgctcatc attttaaaac ccgcatgcag aatttttttg
      121 gccagtttat aatacacggt atccggacgg ttataggtca tcgcgttatc gcacatcagt
      181 ttgaaatcgg ctttaaattc ggtcacgctt ttatattcat tcgccacgat tttatctttc
      241 atggtaccaa aatccatcgg atgtttaata atcatgctat aacccggcgc aatcgcatcg
      301 gtcaccggga acgcaaaaaa gccatgcgga tctttacgct gcagctggcg caggaaatgt
      361 tccagcagct gctgaatcgg ggtgctttca ttttccgcgc tcagtttcag catggattgg
      421 aagtacaggt tctcggtacc cagatctaca ccagaagaat gatgatgatg atggtgcata
      481 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      541 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
      601 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      661 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      721 cgtgagtatg gtggcaggcc ccgtggccgg gggactgttg gggcgccatc tccttgcatg
      781 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
      841 atgcaggagt cgcat
//
