LOCUS       sequence-53331-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(428..448)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(473..490)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             501..523
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    538..562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(562..581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(563..581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(665..684)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tggtggtggt ggtggtgctc gagtgcggcc gcatgcttgt cgacggagct cgaattcgga
       61 tccgtatcca cctttactgt cattcatggt gttcgatttc cgcttttttc atataaaaaa
      121 tttttttaat gctattcgca tctttaaaca cctggctacc cggttcatta taggttttcg
      181 catttttcgc cagcagatca atatctttcg ccatggcgtg gatgctttta tagctaccgt
      241 tctggatacg ctgggcaatg gttttcagat caatcggttc tttaatgatc gcataataat
      301 ccggatactg cactttgctc ggcagtttct gaaacagttc gctgatcaga cgaccgctcg
      361 gattggtcgc aacaacgatg gcttccagca gctgttccag aatttctttc agatacgccg
      421 ggctcatgga ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat
      481 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga
      541 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      601 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      661 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      721 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccggggactg ttggggcgcc
      781 atctccttgc atgcaccatt cccttgcggc ggcggtgctc aacggcctca accctactac
      841 tggggctgct ttccctaatg gca
//
