LOCUS       sequence-53332-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TAF1L
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(511..531)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(556..573)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             584..606
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    621..645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(748..767)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgtagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc aatccaggct
      121 ttccagttcg gcttcttcca gcgccgcttc tttcgcggtg caaatatctt tttccagctg
      181 ggtcagatgt tcatcatatt cggtaatggt ctgatagcaa atattcacaa tttcctgcgc
      241 ggttttggta tactggcttt ccggaccgtt atatttaacg ctgttggcca gaatcaggtt
      301 cacatcatcc agaaagcttt cgcggctctg atatttatgt ttgctaatat ttttacggat
      361 ggtttccaga tcaaccggat tcacaatcat tttataataa tccggaacaa attttttgtt
      421 aaccggatga tgaaacggcc agctatccgg caccgccatc attttctggg tcacgatatt
      481 atccaggata aagctaaagg ccacctgcat ggattggaag tacaggttct cggtacccag
      541 atctacacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta
      601 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      661 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      721 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      781 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcagg
//
