LOCUS       sequence-53338-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MDP1
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(497..517)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(542..559)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             570..592
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acaaaggccc agtttgggcc ttcgcaaatg tctctaaccc ttgacttaga gtttgaagat
       61 tcattccatt ctggatgtga atgcaggtaa cacccagttt gctgacgtct acaatattcc
      121 gcctctcatc atcaaagaag atcatctggg agaaaggaat tccagtcttc tgctgcaacc
      181 tctcaaagtg tgtgatcttg ctgcctggat agatttcccg atgaacaaag tacctgaaga
      241 ggtcaaagag ctccagtagc tggttggccc cttctatctc acttgtcctt gaagcagccg
      301 caccgggcac cccaaggctc tgcaatcgtt ttaggacctc aggcacctct gggtacagtc
      361 ggacgtcttg gccccgccta tctcgtacag ttccatcact gctcttatgg aacggagggt
      421 ctacgtgcgt gtcgacccag aaaggccaga gagtgtaatc caaatcaaag actgccagct
      481 tcggtagccg cgccatggat tggaagtaca ggttctcggt acccagatct acaccagaag
      541 aatgatgatg atgatggtgc atatgtatat ctccttctta aagttaaaca aaattatttc
      601 tagaggggga attgtttatc cgctcacaaa ttccccctaa tagtgaagtc ggtatttaa
//
