LOCUS       sequence-53339-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EP300
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(418..438)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(463..480)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             491..513
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    528..552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(552..571)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(553..571)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(655..674)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttgtcgac ggagctcgaa ttcggatccg tatccacctt tactgtcagc ccaggctctg
       61 catcaccgga tcaatttcct gttcaaacac ttcgctcagt ttgctacaat atttataaac
      121 gcggctggtt ttgcgattat acagccacgc attattaaac atcagccaaa tatcatcaac
      181 atactgccac ggttcctgat actggccggt atccagttta cgtttaatgg tgctcagatc
      241 catcgggctt ttaacaatat caaaataatc cgggataccc agcagctgcg gatccaccgg
      301 ctggcgaaac ggcaggcttt ccggatcctg acgatacagg gcttccaggg tcggcatcag
      361 ggcctgacgc agttcttccg gtttgaaaat tttttttttg ctctggcccg gcgccatgga
      421 ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg
      481 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      541 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
      601 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      661 tcgccgacat caccgatggg gaagatcggg gctcgccact tcgggctcat gagcgcttgt
      721 ttcggcgtgg gtatggtggc aggccccgtg gccgggggga ctgttgggcg ccatctcctt
      781 gcatgcacca ttccttgcgg cggcgggtgc tcaacggccc tcaaccctac tactggggct
      841 ggcttcctaa atg
//
