LOCUS       sequence-53342-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TTC5
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(495..515)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(540..557)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             568..590
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    605..629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccctgaggct tcccattcac cactagcagg aggggcgtct ccactcgaac actggaaaag
       61 gaatagtcct ttcctttgtg ctgaattcgg tgaagccgca ggttgggctc aggaatggct
      121 acagagtctc caatgagcac tccccagctc tgcactatat tgtacaccat cactgcatag
      181 caaggtccat ctgaatctac caggccaaat gtaaagggga ctttctcctc tgtggtgagg
      241 ctaaatacca cctttcccag gatgacggca ccgctgttca ccccaggctg aagcgtactc
      301 agtggcttga gctccagggt cactttctgc ccagaggctg actgatagtg cccatcactg
      361 caagggccta gatgggctgg gcgcaagctt cccagcatgc tctgcagctt tttggtcttc
      421 acctttccct tactctcaag gaggctggtt aatctatcca ggaattccag aagttgttgc
      481 tctcgttgcc gcatggattg gaagtacagg ttctcggtac ccagatctac accagaagaa
      541 tgatgatgat gatggtgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta
      601 gaggggaatt gttatccgct cacaattccc cctatagtgg agtcg
//
