LOCUS       sequence-53345-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ADA
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..25)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(75..98)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(99..116)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(117..137)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc tagcttgtcg acggagctcg
       61 aattcggatc ctcacttatc gtcgtcatcc ttgtaatcat ggtggtgatg atggtgcgat
      121 tggaagtaga ggttctctgc gaggttctgc cctgcagagg ctgaaggtgg catcccatag
      181 gctttataga gcaggtcgag aagctccctc ttttcatctt ctgggaggaa actagatttg
      241 gccgcattga tgttcagcct tttaaactcc tcttcagtaa agcccatgtc ccgtttggtc
      301 atctggtaat cagtgtccag ggtggacttg aagatgagcg ggtcatctgt gttgagcgag
      361 tagttagcct ggtcattttt gagccgaatg actgcatgct ccgtgtccgg cttccaggca
      421 ccagtgaggt agctggacca ggggcagatc tcgaagtgca tgttttcctg ccgcagcctg
      481 ttataaaggg cctggtcttc cagggtgtgg tagccgtgtc ccagccgctc tgtcttgagt
      541 atgtccacag cctcttttac tacttcggcc gagcccacct ccccggcgtg gacagtacgg
      601 tgaatgccgc tcttcacagc ctcctggtag gcctggacat gtccaggcaa gaggctgctt
      661 cctgggatgg tctcatctcc agccaggtca atggctacca cggtctgctg ctggtacttc
      721 ttacacagct ccaccacctt gggggaccag ttgggctggt ggcgcatgca gcacaggatg
      781 gaccgggcct tgaccccgaa gtctcgctcc ccctcctgca ggccctggcc cactagggcc
      841 accacctcgt ctggggtgag gtccccttca
//
