LOCUS       sequence-53346-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GOT1
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..88)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(89..106)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(107..127)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggtggtggtg gtggtgctcg agtgcggccg cagcttgtcg acggagctcg aattcggatc
       61 ctcacttatc gtcgtcatcc ttgtaatcat ggtggtgatg atggtgcgat tggaagtaga
      121 ggttctctgc gattttggtg actgcttcat ggatggaggt ggccacgtaa tctagatttt
      181 tggtggttaa gccactcacg ttgattcgac cacttggcag caggtagatg tgcttttcat
      241 tgaccagata ctcaacctgc ttggggttca acccagtgaa gctgaacatg ccaatttgat
      301 cagtgatgtg gttccaggtc ccaggggttt tgagggcttc tagtcgtgcc ctgagttcag
      361 atctcatggt cagaatccgg tcagccattg tcttcacatt acctgtccat tcctcaaaga
      421 gctcagggtt agagagggtg ctggccacaa ttcgtgctcc ctgggcgggg ggattggacc
      481 aagtaatccg cacgatcttc tccatctggg aaaggacttg caggatgctc tcaggttctt
      541 ttccaaccac agtcagattc ccgactctct cattgtagag cccgaagttc ttggagaagg
      601 actgggcaca gaagaactcg aagccttcag acacaaaata gcgaatggcc caggcatctc
      661 tctccaggtt tccagatgcg aagccctgat aggctgagtc aaagaagggg aacagaaacc
      721 ggtgcttcat gaca
//
