LOCUS       sequence-53348-e         588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 588)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SEPX1
FEATURES             Location/Qualifiers
     source          1..588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(374..394)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(419..436)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             447..469
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    484..508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(508..527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(509..527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggcgtcta taagtggtcg aacggagctc gaattcggat ccgtatccac ctttactgtc
       61 aggcagaagt gtctttgcct ttagggacaa acttcagcga gctgctgaat attgagaatc
      121 gggactgccc cggcttgggg ccgtcgttca ggaactcgtg gcccaaccca ttgccacact
      181 tgccacagga caccttcaag gcttcagatc tattgtgctc cggacgcttg gccacgctgt
      241 cggcgtgaat ggtctcggtg aacgccggcc atggagacga gtgtgcatac ttcgagcggc
      301 tggagaacag ctcatagcca cacttggcac acacgtaaac gccaggttca aagtgattct
      361 ggaaaacctt catggattgg aagtacaggt tctcggtacc cagatctaca ccagaagaat
      421 gatgatgatg atggtgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      481 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcggggat
      541 cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccgg
//
