LOCUS       sequence-53351-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CBLB
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgct aatccagtta aatcaggatt ataactcctc
      181 ccatcaggat aaagataaaa tccttccctg ctgccatcaa tcagggcttg aaataagggc
      241 ttgttatgag gtatggtctg taagatattc ccatccccag tcacatagcc aatggcccac
      301 tgtcccaatc gagtgcaact taaccggaaa atatagcttc cgggtttggt gctatatttc
      361 tgtagtcgtg ctttaacttc atcatatgtg agaaatgcca tgtaacctgg atgtgtcaca
      421 gctaagaaat tccaattccg caaaatagag ccccaaggct gaaacagcct ggtaaaaata
      481 tcaaattcaa aaactgaaat gtaatcattg caagttaaat caattgttga ttttagagcc
      541 attgcttcca ggccagagct aatctggtgg acctcatgaa ggcactgtct gaatactttc
      601 catggtacga tagttttgtc tccaaaaaac tttctccaga attcagcagc atctgctttt
      661 gtgatacgaa agttatctcc ctggaattga ccattgggaa agattgcttt gatttctgcc
      721 agcatgtgac tgaagataag ggacagtttt gtgagatttc gtctgtcctg tgactgttct
      781 tcatacattc tctccttgcc ttctttaaag agtcttattg cccgttttga ctttttcata
      841 aggctatcaa tgtagatttt aaagtactca ttctcactga gttgggcaag tttctggttg
      901 tcatcatatt tactcaatat aagtcgtaaa tgctga
//
