LOCUS       sequence-53356-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Goldstein AL, McCusker JH
  TITLE     Three new dominant drug resistance cassettes for gene disruption in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1999 Oct;15(14):1541-53.
  PUBMED    10514571
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1999-10"; volume: "15(14)"; pages: "1541-53"
COMMENT     Sequence Label: pAG31
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..501
                     /codon_start=1
                     /gene="Streptomyces viridochromogenes pat1"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialaphos or phosphinothricin"
                     /translation="MAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVA
                     EVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKS
                     VVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDF"
     terminator      555..752
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 agcccagaac gacgcccggt cgagatccgt cccgccaccg ccgccgacat ggcggcggtc
       61 tgcgacatcg tcaatcacta catcgagacg agcacggtca acttccgtac ggagccgcag
      121 actccgcagg agtggatcga cgacctggag cgcctccagg accgctaccc ctggctcgtc
      181 gccgaggtgg agggcgtcgt cgccggcatc gcctacgccg gcccctggaa ggcccgcaac
      241 gcctacgact ggaccgtcga gtcgacggtg tacgtctccc accggcacca gcggctcgga
      301 ctgggctcca ccctctacac ccacctgctg aagtccatgg aggcccaggg cttcaagagc
      361 gtggtcgccg tcatcggact gcccaacgac ccgagcgtgc gcctgcacga ggcgctcgga
      421 tacaccgcgc gcgggacgct gcgggcagcc ggctacaagc acgggggctg gcacgacgtg
      481 gggttctggc agcgcgactt cgagctgccg gccccgcccc gccccgtccg gcccgtcaca
      541 cagatctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
      601 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
      661 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
      721 tcaatcgtat gtgaatgctg gtcgctatac tgctg
//
