LOCUS       sequence-53363-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHIP
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(507..527)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(552..569)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             580..602
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    617..641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(641..660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(642..660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(744..763)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcaacgttt atgaaagcgc
      121 agcgcgcttt tataatcgct cagcacgctg ctaatgtgtt cttcaaaaaa cgcgctcaga
      181 cgcaggctca tgctatagat acggctacgt ttgctcgggg tataggcttt gctgttgcta
      241 aaaatcaggc gcacatcttt gcacagttcc atcgggcttt catagttacc cgcttccagg
      301 gtttcgcgca cggtcgcaaa atccatcggg gtatcaatga tatcgcgata atccggatat
      361 tccagcagat caaccggctg acgaaacggt tcgctatctt cacactgaaa aatcagattc
      421 agcagttctt cgcactgttt tttccacgcc tggatatcat agctctgcgc acggttgcgc
      481 agacgacgac gcggctgatg atccatggat tggaagtaca ggttctcggt acccagatct
      541 acaccagaag aatgatgatg atgatggtgc atatgtatat ctccttctta aagttaaaca
      601 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta
      661 atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg
      721 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      781 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      841 cggggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
      901 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgt
//
