LOCUS       sequence-53364-e         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GMFG
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(451..471)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(496..513)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    562..586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(586..605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(587..605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcagaaag acaacttttc
       61 ttggagccag gcctcagtga ggtcatcagt ggtgcggatt tcgaacacct ttgtgagctc
      121 tgctgtctgc accagcctgt ttttactccc tgcatacatc atctgttgtt ccggcttgca
      181 gcccacaggg ctggagaaga tgaaacacaa agggtaggac actcggccat cgtcatgcac
      241 gtacttgtag ctgtaaacca cgaacctggg ctgtctctcc ggcaactcca ttttgagctc
      301 ctctggggaa atgttctgaa attcttcctc cagcaccacc atctgccggt ctttgtccac
      361 cttcattatg atggctgcat tgtctgtctc ttttcggaag cggaatttcc tcagcttttc
      421 tgttagctct gggtctacct cgcacaccat ggattggaag tacaggttct cggtacccag
      481 atctacacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta
      541 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      601 tattaatttc gcgggatcga gatctcg
//
