LOCUS       sequence-53365-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(426..446)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(471..488)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             499..521
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    536..560
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(560..579)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(561..579)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggtggtggt ggtgctcgag tgcggctgca tgtaatgtcg acggagctcg aattcggatc
       61 cgtatccacc tttactgtca ttcacctttc tgcacaaatt cattgcgggt acgcagatac
      121 agatcccaca gtttgcacgc ggctttatat tccgggctat ccggtttata atagcttttc
      181 gcattattaa acagcagctg aaaatccgcg gtcagcaggt tcacatcatc atattcttcc
      241 attttcagtt tctgctgaat tttcatcaga tcgatcggct ggctaaccac ttcataataa
      301 tccggctgat tgcgacgttt cggggcgcga atgaacagtt cgcacagcag gcgaccctgt
      361 tcatctttat aatcgcgaat ggtattatac agttcatgac acacggcaat cggatcaacg
      421 gtcatggatt ggaagtacag gttctcggta cccagatcta caccagaaga atgatgatga
      481 tgatggtgca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
      541 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      601 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cgg
//
