LOCUS       sequence-53366-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Kim SH, Kim K, Kwagh JG, Dicker DT, Herlyn M, Rustgi AK, Chen Y, 
            El-Deiry WS
  TITLE     Death induction by recombinant native TRAIL and its prevention by a 
            caspase 9 inhibitor in primary human esophageal epithelial cells.
  JOURNAL   J Biol Chem. 2004 Sep 17. 279(38):40044-52.
  PUBMED    15226295
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Sep 17. 279(38):40044-52."
COMMENT     Sequence Label: pQE-hTR
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..30
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(54..68,69..74,75..91,92..98)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    74..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             141..152
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             171..188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      831..865
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ttaacctata aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa
       61 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
      121 aatttcacac agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc
      181 accatcacgg atccacctct gaggaaacca tttctacagt tcaagaaaag caacaaaata
      241 tttctcccct agtgagagaa agaggtcctc agagagtagc agctcacata actgggacca
      301 gaggaagaag caacacattg tcttctccaa actccaagaa tgaaaaggct ctgggccgca
      361 aaataaactc ctgggaatca tcaaggagtg ggcattcatt cctgagcaac ttgcacttga
      421 ggaatggtga actggtcatc catgaaaaag ggttttacta catctattcc caaacatact
      481 ttcgatttca ggaggaaata aaagaaaaca caaagaacga caaacaaatg gtccaatata
      541 tttacaaata cacaagttat cctgacccta tattgttgat gaaaagtgct agaaatagtt
      601 gttggtctaa agatgcagaa tatggactct attccatcta tcaaggggga atatttgagc
      661 ttaaggaaaa tgacagaatt tttgtttctg taacaaatga gcacttgata gacatggacc
      721 atgaagccag ttttttcggg gcctttttag ttggctaaaa gcttaattag ctgagcttgg
      781 actcctggtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
      841 tcggttgccg ccgggcgttt tttattggtg agaatccaag ctag
//
