LOCUS       sequence-53369-e        7504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW
  TITLE     Sds22 and Repo-Man stabilize chromosome segregation by counteracting
            Aurora B on anaphase kinetochores.
  JOURNAL   J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16.
  PUBMED    22801782
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-07-23"; volume: "198(2)"; pages: "173-83. Epub 
            2012 Jul 16"
COMMENT     Sequence Label: pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-pu...
FEATURES             Location/Qualifiers
     source          1..7504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(69..71,72..785)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     intron          855..1084
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1067..1086
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(1142..1716,1717..1719,1720..1728)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1309..1326)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1536..1555
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1748..2347
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1748..1767)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2244..2264
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2483..2500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2489..2713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2760..2779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2839..2855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2839..2855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2852..2874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2863..2879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2887..2893,2894..2911,2912..2917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2932..2953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3070..3087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3241..3829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3321..3340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4000..4791,4792..4860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4623..4642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4861..4965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5040..5059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5231..5610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5611..5814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5765..5785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5859..5878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5859..5877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5902..6279
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6292..6301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6298..6300,6301..6303,6304..7014)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(6298..6300,6301..6303,6304..7014)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(6298..6300,6301..6303,6304..7014)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
ORIGIN
        1 gatccggcgg caaggtgaac aagatcgtga agaaccgccg caccgtggcc atcggtggtt
       61 ccggaggagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      121 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      181 cctacggcaa gctgaccctg aagctgctct gcaccaccgg caagctgccc gtgccctggc
      241 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
      301 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      361 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      421 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      481 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
      541 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      601 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      661 accactacct gagctaccag tccgccctgt tcaaagaccc caacgagaag cgcgatcaca
      721 tggtcctgct ggagttcctg accgccgccg ggatcactga aggcatgaac gagctgtaca
      781 agtaaagcgg ccgcatagat aactgatcca gtgtgctgga attaattcgc tgtctgcgag
      841 ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt
      901 gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag
      961 ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg
     1021 caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1081 acaggtgtcc actcccaggt ccaactgcag gtcgagcatg catctagggc ggccaattcc
     1141 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     1201 gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat gtgagggccc
     1261 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     1321 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     1381 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     1441 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     1501 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     1561 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     1621 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     1681 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg
     1741 accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     1801 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     1861 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     1921 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     1981 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     2041 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     2101 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     2161 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     2221 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     2281 caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg
     2341 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg
     2401 ctccgaccga agccgacccg ggcggccccg ccgaccccgc acccgccccc gaggcccacc
     2461 gactctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2521 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2581 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2641 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2701 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgagtgc attctagttg
     2761 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     2821 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2881 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2941 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3001 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3061 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3421 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4981 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc gactctcagt
     5041 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     5101 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     5161 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     5221 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     5281 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5341 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5401 ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg
     5461 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     5521 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     5581 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     5641 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5701 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     5761 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     5821 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     5881 acccaagctt ggcgcgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag
     5941 ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc
     6001 cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc
     6061 ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc
     6121 atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg
     6181 gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc
     6241 gagggtacta aggccatcac caagtacacc agcgctaagc tagcaagctt cgccaccatg
     6301 gtgagcaagg gcgaggagct gttcggcggg atcgtgccca tcctggtcga gctggagggc
     6361 gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     6421 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     6481 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     6541 cacgacttct tcaagtccgt catgcccgaa ggctacgtcc aggagcgtac catcttcttc
     6601 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     6661 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     6721 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     6781 atcaaggccc acttcaaggc ccgccacaac atcacggacg gcagcgtgca gctcgccgac
     6841 cactaccagc agaacacccc catcggcgac ggccccgtga tcctgcccga caaccactac
     6901 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     6961 ctggagttcg tgaccgccgc cgggatcact cacggcatgg acgagctgta caaggctggt
     7021 accaagggta atggtaggtt tttaacttta aaaccattgc ctgacagcat tattcaagaa
     7081 agcctggaga ttcagcaagg tgtgaatcca tttttcattg gtagatccga ggattgcaat
     7141 tgtaaaattg aagacagtag gttgtctcga gttcattgct tcattttcaa aaagaggcat
     7201 gctgtaggca aaagcatgta tgaatctccg gcacaaggtt tagatgatat ttggtattgc
     7261 cacaccggaa ctaacgtgag ctatttaaat aataaccgca tgatacaggg tacgaaattc
     7321 cttttacaag acggagatga aatcaagatc atttgggata aaaacaataa atttgtcatt
     7381 ggctttaaag tggaaattaa cgatactaca ggtctgttta acgagggatt aggtatgtta
     7441 caagaacaaa gagtagtact taagcaaaca gccgaagaaa aagatttggt gaaaaagtta
     7501 ggag
//
