LOCUS       sequence-53372-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAST3
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(336..356)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(381..398)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             409..431
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    446..470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(470..489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(471..489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(573..592)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gacggagctc gaattcggat ccgtatccac ctttactgtc aaacgctggt ttcggtattt
       61 tccagcgcgg tggtacgcag gctaatttta ttgccgcttt tcagcagcag ttccaccaca
      121 tccatgtgaa ccaggcccag cacgctttcg ccgttaatat gggtaatcag atcgcccgca
      181 cgcaggcccg cttcctgggc cgggctgcca tcttccacgc tccacaccac atgatgaacg
      241 gtataaacat cgctatcacc catatacacg cgaatggcgc gcaggctaaa gccatatttt
      301 ttgccgctgc tatgaatcac aatcggcgga cgcatggatt ggaagtacag gttctcggta
      361 cccagatcta caccagaaga atgatgatga tgatggtgca tatgtatatc tccttcttaa
      421 agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga
      481 gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg
      541 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      601 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      661 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      721 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataa
//
