LOCUS       sequence-53378-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FDX1
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..48)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(49..66)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(67..87)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    509..533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(533..552)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(534..552)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(636..655)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 acggagctcg aattcggatc ctcacttatc gtcgtcatcc ttgtaatcat ggtggtgatg
       61 atggtgcgat tggaagtaga ggttctctgc ggaggtcttg cccacatcaa tggattgtct
      121 ggcatcagcc actgtttcag gcactcgaac agtcatattg tccatagatt ttgtcaaaca
      181 gatttggcag cccaaccgtg atctgtctgt tagtccatat gccagatcga gcatgtcatt
      241 ctcctcatca gtgattgcat ctaacttctc atatatgtga tcttcaaaga tgaggtgaca
      301 ggttgaacaa gccagggttc cctcacatgc accaaagcca tcaatatcta gattattttc
      361 aaccacaaca tctagcagag aatcaccaac ttttcctttg gttgttaatg tttcaccatc
      421 acggtttata aagtggactg ttattttatc ttctgagctg ctcatagtat atctccttct
      481 taaggttaaa caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag
      541 tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc
      601 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      661 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtg
//
