LOCUS       sequence-53379-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RAMP2
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(324..344)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(369..386)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             397..419
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    434..458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(458..477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(459..477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(561..580)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcaagagaa ggtgggctgc
       61 accagggagc agttggcaaa gtggatctgg tgagtctcaa agatgatcct ctctgccaag
      121 ggattgggga agcccaggtc aaacaactct gcaaagtgct ccaggcaatc tcgcagggtg
      181 ctataaggcc tgctaatcat ggcccagtcg caccaatcct tttcgatagg atccatttga
      241 tccttataat gattccagca aaattggaca gctgtctcat agttcttcac cgtcccccct
      301 tctgaccctg gtgtgcctgt catggattgg aagtacaggt tctcggtacc cagatctaca
      361 ccagaagaat gatgatgatg atggtgcata tgtatatctc cttcttaaag ttaaacaaaa
      421 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      481 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      541 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      601 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      661 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      721 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaaa
      781 tccccggaca ccatccgaat g
//
