LOCUS       sequence-53383-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1A3
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    82..106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             121..143
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..507
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             514..534
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcgcggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa
       61 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
      121 tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt
      181 atgagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg
      241 gacggggcga tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc
      301 ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac
      361 atcgatcaaa accctggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg
      421 ctgttcaaaa acggtgaagt ggcggcaacc aaagtgggcg cactgtctaa aggtcagttg
      481 aaagagttcc tcgacgctaa cctggccggt accgagaact tgtacttcca atccatgcaa
      541 ggtcaggtcg ttcaagctaa gaaattcagc aatgtgacga tgctgtttag tgatatcgta
      601 gggttcaccg ccatttgctc acagtgttcc ccgctgcagg tcatcactat gctgaacgca
      661 ctgtacacac gctttgacca acagtgcggc gaactggatg tttataaagt ggagacaatt
      721 ggtgacgcgt actgtgtagc tggcggtctg cataaggaaa gcgataccca cgccgtccaa
      781 atcgcactga tggcgctgaa aatgatggaa ctgtcggatg aggttatgtc tccgc
//
